Plant Transcription Factor Database
Previous version: v3.0
Trifolium pratense
HSF Family
Species TF ID Description
Tp57577_TGAC_v2_mRNA10587HSF family protein
Tp57577_TGAC_v2_mRNA11110HSF family protein
Tp57577_TGAC_v2_mRNA12197HSF family protein
Tp57577_TGAC_v2_mRNA12237HSF family protein
Tp57577_TGAC_v2_mRNA12415HSF family protein
Tp57577_TGAC_v2_mRNA141HSF family protein
Tp57577_TGAC_v2_mRNA14168HSF family protein
Tp57577_TGAC_v2_mRNA14807HSF family protein
Tp57577_TGAC_v2_mRNA16976HSF family protein
Tp57577_TGAC_v2_mRNA19776HSF family protein
Tp57577_TGAC_v2_mRNA19938HSF family protein
Tp57577_TGAC_v2_mRNA19939HSF family protein
Tp57577_TGAC_v2_mRNA23619HSF family protein
Tp57577_TGAC_v2_mRNA25037HSF family protein
Tp57577_TGAC_v2_mRNA26238HSF family protein
Tp57577_TGAC_v2_mRNA26601HSF family protein
Tp57577_TGAC_v2_mRNA27011HSF family protein
Tp57577_TGAC_v2_mRNA3667HSF family protein
Tp57577_TGAC_v2_mRNA3668HSF family protein
Tp57577_TGAC_v2_mRNA3844HSF family protein
Tp57577_TGAC_v2_mRNA5882HSF family protein
Tp57577_TGAC_v2_mRNA6888HSF family protein
Tp57577_TGAC_v2_mRNA6992HSF family protein
Tp57577_TGAC_v2_mRNA7357HSF family protein
Tp57577_TGAC_v2_mRNA8362HSF family protein
Tp57577_TGAC_v2_mRNA9782HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645